LOCUS       3                       9710 bp    DNA     circular SYN 23-SEP-2025
DEFINITION  3.
ACCESSION   3
VERSION     3
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        5212..6390
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="strong constitutive promoter for human
                     elongation factor EF-1α"
     CDS             6409..7155
                     /note="pLannotate"
                     /label="Tet-On® 3G"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     rep_origin      8094..8522
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis "
     promoter        complement(4663..5030)
                     /note="pLannotate"
                     /label="TRE3GS promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="3rd-generation Tet-responsive promoter that
                     can be activated by binding of Tet-On® 3G, modified to
                     eliminate binding sites for endogenous mammalian
                     transcription factors"
     CDS             complement(1915..2706)
                     /note="pLannotate"
                     /label="KKA2_KLEPN"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Resistance to kanamycin,
                     neomycin, paromomycin, ribostamycin, butirosin and
                     gentamicin B. From Klebsiella pneumoniae."
     CDS             8680..9540
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     CDS             complement(3607..4632)
                     /note="pLannotate"
                     /label="ETV2_HUMAN"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at transcript level:
                     Swiss-Prot protein existence level 2. Binds to DNA
                     sequences containing the consensus pentanucleotide
                     5'-CGGA[AT]-3'. From Homo sapiens (Human)."
     polyA_signal    complement(1617..1738)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     promoter        8575..8679
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     misc_feature    complement(5034..5125)
                     /note="pLannotate"
                     /label="pause site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /description="RNA polymerase II transcriptional pause
                     signal from the human α2 globin gene"
     polyA_signal    7272..7353
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     promoter        complement(2781..2991)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="SV40 early promoter"
     polyA_signal    complement(3344..3399)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="rabbit β-globin polyadenylation signal (Gil
                     and Proudfoot, 1987)"
     polyA_signal    complement(5139..5187)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="synthetic polyadenylation signal"
     CDS             7932..8089
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
                     /description="LacZα fragment of β-galactosidase; lacZ
                     fragment"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for the E. coli lac operon"
     promoter        1490..1520
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for the E. coli lac operon"
     protein_bind    complement(7384..7408)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    3579..3603
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="CAP binding activates transcription in the
                     presence of cAMP. E. coli catabolite activator protein"
     protein_bind    complement(4638..4658)
                     /note="pLannotate"
                     /label="attB5"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; core recombination
                     site for the Gateway® BP reaction BP Clonase™"
     promoter        complement(7911..7929)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T7 RNA polymerase"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="The lac repressor binds to the lac operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG). lac repressor
                     encoded by lacI"
     protein_bind    1528..1544
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="The lac repressor binds to the lac operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG). lac repressor
                     encoded by lacI"
     CDS             complement(1733..1891)
                     /note="pLannotate"
                     /label="BLE_KLEPN (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="42.1"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binding protein with a strong
                     affinity to the bleomycin family of antibiotics. Binds to
                     CL990; an antimitotic-antibiotic compound. From Klebsiella
                     pneumoniae."
     CDS             complement(3470..3547)
                     /note="pLannotate"
                     /label="HBB_PONPY (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="72.2"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at transcript level:
                     Swiss-Prot protein existence level 2. Involved in oxygen
                     transport from the lung to the various peripheral tissues.
                     From Pongo pygmaeus (Bornean orangutan)."
     CDS             complement(1047..1178)
                     /note="pLannotate"
                     /label="FP25_NPVAC (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. From Autographa californica
                     nuclear polyhedrosis virus (AcMNPV)."
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
                     /description="lac repressor; lacI; The lac repressor binds
                     to the lac operator to inhibit transcription in E. coli.
                     This inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     repeat_region   7364..7375
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     repeat_region   complement(749..760)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     CDS             7166..7249
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
                     /description="large T antigen from SV40 (simian virus 40)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc tttagcgcgc
     1081 gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc tatgacgtgt
     1141 ttaaagttta ggtcgagtaa agcgcaaatc ttttttaacc ctagaaagat agtctgcgta
     1201 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
     1261 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
     1321 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
     1381 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
     1441 acttacgtga taacttatta tatatatatt ttcttgttat agatcaggct ttacacttta
     1501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1561 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     1621 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1681 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     1741 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     1801 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     1861 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     1921 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     1981 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     2041 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     2101 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     2161 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     2221 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     2281 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     2341 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     2401 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     2461 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     2521 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     2581 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     2641 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     2701 aatcatgcga aacgatcctc atcctgtctc ttgatcagat ccgaaaatgg atatacaagc
     2761 tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     2821 tagctcagag gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     2881 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     2941 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg cctggatcga
     3001 attcctgcag gtcgagggat ctccataaga gaagagggac agctatgact gggagtagtc
     3061 aggagaggag gaaaaatctg gctagtaaaa catgtaagga aaattttagg gatgttaaag
     3121 aaaaaaataa cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag gcttttctat
     3181 ggaataagga atggacagca gggggctgtt tcatatactg atgacctctt tatagccaac
     3241 ctttgttcat ggcagccagc atatgggcat atgttgccaa actctaaacc aaatactcat
     3301 tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga cacaaaaaat
     3361 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa
     3421 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
     3481 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
     3541 tgaattcgat gggggatccc ttcgctagag cggccatcac cactttgtac aagaaagctg
     3601 ggtttattgt gtctctgctc cccgtccgcc tcccgcacag tccggatagg ctaggctggg
     3661 cacgcggccc ccgaagcggt acgtgtactt tcgccccccg ctcttgcgca cgatgtcgcg
     3721 gcgatagtag tagcgaaggc cccggctcag cttctcgtaa ttcatgcccg gctttctctt
     3781 gcgctcgccc cacagccgag ccacctcttt ggggtcgcac agctggaact cgcggctgtt
     3841 gccagtccaa cggatgcagc tgctacgcgc cccgtcgtgg agcagctcca ggaggaactg
     3901 ccacagctga atgggacctc ggtggttagt tttggggcat cgagccaaac tggcacggtc
     3961 cgactgcggg ctcggttcgg agcaaacggt gagagctgag ctctggtacc gcttcaaaga
     4021 ggtggtgcca cccgcatgca gccccgggtt ccaggaggtg gtacagtccg ggcccgcggg
     4081 cccgccccac gaaatggtac agtccgtgcg cggctccccg cccaggccac tgccccagta
     4141 ggtatcgccg tcgggcccca cagaacagtc ccagctggtg ttgctcccgg cggcctgggc
     4201 gcgcgaccac gaggtggcct ctcccgccac ggggacgcag ttctggcccg cggcgccttc
     4261 ggagccggcg gcggggatgg ggcccgggcc gagaggggcg gggcccaggg tctgcgaggc
     4321 gccgctccaa gagtcccagg ctgtgcacgc catgtctgtc cagtccccgg accacggaag
     4381 agcctgagag tcgggctccg ccccccatgg aacttctggg tgcagtaacg cggagcccca
     4441 gtccagctgt gggaagcttg ccagggatga gcttgtacct ttccagcatg tctctgctgt
     4501 cgctgtcggc gtgtcccctt ggagtgccag atcagggaaa cagaagccta atttggctcc
     4561 ttcaagccct gccagcttgt tccctggagg cacttcctgt ggggatgcct catcccagtt
     4621 ccacaggtcc atggtggcaa cttttgtata caaagttgat tctttacgag ggtaggaagt
     4681 ggtacggaaa gttggtataa gacaaaagtg ttgtggaatt gaagtttact caaaaaatca
     4741 gcactctttt ataggcgccc tggtttacat aagcaaagct tatacgttct ctatcactga
     4801 tagggagtaa actggatata cgttctctat cactgatagg gagtaaactg tagatacgtt
     4861 ctctatcact gatagggagt aaactggtca tacgttctct atcactgata gggagtaaac
     4921 tccttatacg ttctctatca ctgataggga gtaaagtctg catacgttct ctatcactga
     4981 tagggagtaa actcttcata cgttctctat cactgatagg gagtaaactc gagagagaaa
     5041 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
     5101 tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag
     5161 atgtaatgaa aataaagata ttttattctc gaggtgataa ttccaccgtg aggctccggt
     5221 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5281 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5341 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5401 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     5461 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     5521 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     5581 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     5641 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     5701 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     5761 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     5821 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     5881 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     5941 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     6001 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     6061 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     6121 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     6181 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     6241 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     6301 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     6361 agtttttttc ttccatttca ggtgtcgtga ggggatcatc gaattaccat gtctagactg
     6421 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc
     6481 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     6541 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     6601 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     6661 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     6721 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     6781 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     6841 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     6901 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct
     6961 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     7021 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     7081 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     7141 atgctccccg ggtaaatctc cagaggatca taatcagcca taccacattt gtagaggttt
     7201 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7261 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7321 caaatttcac aaataaagca tttttttcac tgccccgagc ttcctcgctc actgactcgc
     7381 tgcagcctgc ttttttgtac aaacttgtga tgccgccacc gcggtagcac tagttagctt
     7441 aattaacgag agcataatat tgatatgtgc caaagttgtt tctgactgac taataagtat
     7501 aatttgtttc tattatgtat aggttaagct aattacttat tttataatac aacatgactg
     7561 tttttaaagt acaaaataag tttatttttg taaaagagag aatgtttaaa agttttgtta
     7621 ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa
     7681 ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt
     7741 caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca
     7801 tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaat aatagtttct
     7861 aattttttta ttattcagcc tgctgtcgtg aataccgagc tccaattcgc cctatagtga
     7921 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7981 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8041 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8101 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8161 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8221 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8281 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8341 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8401 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8461 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8521 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8881 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
