LOCUS       2                       9792 bp    DNA     circular SYN 23-SEP-2025
DEFINITION  2.
ACCESSION   2
VERSION     2
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        5294..6472
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="strong constitutive promoter for human
                     elongation factor EF-1α"
     CDS             6491..7237
                     /note="pLannotate"
                     /label="Tet-On® 3G"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     rep_origin      8176..8604
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis "
     CDS             complement(1987..2385)
                     /note="pLannotate"
                     /label="BSD"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="blasticidin S deaminase; Aspergillus terreus
                     BSD; confers resistance to blasticidin"
     promoter        complement(4745..5112)
                     /note="pLannotate"
                     /label="TRE3GS promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="3rd-generation Tet-responsive promoter that
                     can be activated by binding of Tet-On® 3G, modified to
                     eliminate binding sites for endogenous mammalian
                     transcription factors"
     CDS             8762..9622
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     CDS             complement(3257..4690)
                     /note="pLannotate"
                     /label="ERG_HUMAN"
                     /database="swissprot"
                     /identity="99.6"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Transcriptional regulator. May
                     participate in transcriptional regulation through the
                     recruitment of SETDB1 histone methyltransferase and
                     subsequent modification of local chromatin structure. From
                     Homo sapiens (Human)."
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     promoter        8657..8761
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     misc_feature    complement(5116..5207)
                     /note="pLannotate"
                     /label="pause site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /description="RNA polymerase II transcriptional pause
                     signal from the human α2 globin gene"
     polyA_signal    7354..7435
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     promoter        complement(2415..2477)
                     /note="pLannotate"
                     /label="Mini-TK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="minimal herpes simplex virus (HSV) thymidine
                     kinase promoter"
     polyA_signal    complement(2994..3049)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="rabbit β-globin polyadenylation signal (Gil
                     and Proudfoot, 1987)"
     polyA_signal    complement(5221..5269)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="synthetic polyadenylation signal"
     polyA_signal    complement(1889..1937)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             8014..8171
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
                     /description="LacZα fragment of β-galactosidase; lacZ
                     fragment"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for the E. coli lac operon"
     protein_bind    complement(7466..7490)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    3229..3253
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="CAP binding activates transcription in the
                     presence of cAMP. E. coli catabolite activator protein"
     protein_bind    complement(4720..4740)
                     /note="pLannotate"
                     /label="attB5"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; core recombination
                     site for the Gateway® BP reaction BP Clonase™"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(7993..8011)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="The lac repressor binds to the lac operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG). lac repressor
                     encoded by lacI"
     CDS             complement(3120..3197)
                     /note="pLannotate"
                     /label="HBB_PONPY (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="72.2"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at transcript level:
                     Swiss-Prot protein existence level 2. Involved in oxygen
                     transport from the lung to the various peripheral tissues.
                     From Pongo pygmaeus (Bornean orangutan)."
     CDS             complement(1047..1178)
                     /note="pLannotate"
                     /label="FP25_NPVAC (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. From Autographa californica
                     nuclear polyhedrosis virus (AcMNPV)."
     CDS             complement(1489..1689)
                     /note="pLannotate"
                     /label="GH_HHV11 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. The heterodimer glycoprotein
                     H-glycoprotein L is required for the fusion of viral and
                     plasma membranes leading to virus entry into the host cell.
                     Following initial binding to host receptor, membrane fusion
                     is mediated by the fusion machinery composed of gB and the
                     heterodimer gH/gL. May also be involved in the fusion
                     between the virion envelope and the outer nuclear membrane
                     during virion morphogenesis. From Human herpesvirus 1
                     (strain 17) (HHV-1)."
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
                     /description="lac repressor; lacI; The lac repressor binds
                     to the lac operator to inhibit transcription in E. coli.
                     This inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             2451..2642
                     /note="pLannotate"
                     /label="UL24_EHV4 (fragment)"
                     /database="swissprot"
                     /identity="50.0"
                     /match_length="23.5"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at transcript level:
                     Swiss-Prot protein existence level 2. May participate in
                     nuclear egress of viral particles. Plays a role in the
                     dispersal of several host nucleolar proteins including
                     NCL/nucleolin and NPM1. Since deletion of host
                     NCL/nucleolin negatively impact on nuclear egress, UL24
                     supposedly acts on this process through its effect on host
                     nucleoli (By similarity). From Equine herpesvirus 4 (strain
                     1942) (EHV-4)."
     repeat_region   7446..7457
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     repeat_region   749..760
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     CDS             7248..7331
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
                     /description="large T antigen from SV40 (simian virus 40)"
     misc_feature    complement(2643..2660)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="16.7"
                     /fragment="True"
                     /other="misc_feature"
                     /description="pBluescript multiple cloning site"
     polyA_signal    1782..1798
                     /note="pLannotate"
                     /label="bGH poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="polyA_signal"
                     /description="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc tttagcgcgc
     1081 gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc tatgacgtgt
     1141 ttaaagttta ggtcgagtaa agcgcaaatc ttttttaacc ctagaaagat agtctgcgta
     1201 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
     1261 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
     1321 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
     1381 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
     1441 acttacgtga taacttatta tatatatatt ttcttgttat agatatcgcg ccagaaatcc
     1501 gcgcggtggt ttttgggggt cgggggtgtt tggcagccac agacgcccgg tgttcgtgtc
     1561 gcgccagtac atgcggtcca tgcccaggcc atccaaaaac catgggtctg tctgctcagt
     1621 ccagtcgtgg acctgacccc acgcaacgcc caaaataata acccccacga accataaacc
     1681 attccccatg ggggaccccg tccctaaccc acggggccag tggctatggc agggcctgcc
     1741 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa
     1801 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc
     1861 cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt
     1921 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     1981 agatctttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     2041 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     2101 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     2161 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     2221 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     2281 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     2341 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatatcag atctgcggca
     2401 cgctgttgac gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg
     2461 tcaccttaat atgcgaagtg gacctcggac cgcgccgccc cgactgcatc tgcgtgttcg
     2521 aattcgccaa tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca
     2581 aatatatttc ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc
     2641 aggatatcga attcctgcag gtcgagggat ctccataaga gaagagggac agctatgact
     2701 gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga aaattttagg
     2761 gatgttaaag aaaaaaataa cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag
     2821 gcttttctat ggaataagga atggacagca gggggctgtt tcatatactg atgacctctt
     2881 tatagccaac ctttgttcat ggcagccagc atatgggcat atgttgccaa actctaaacc
     2941 aaatactcat tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga
     3001 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc
     3061 agatgctcaa ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag
     3121 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
     3181 acctgaggag tgaattcgat gggggatccc ttcgctagag cggccatcac cactttgtac
     3241 aagaaagctg ggtttagtag taagtgccca gatgagaagg catatggctg gtggggagcc
     3301 tagtgttggg gtatataccc ccagttggtg aattccagta tgggtttggg gcagcaaaaa
     3361 aactggaaga tgtcacgggg agggctggag ggtggggcgc cacaaagttc atcttctgtg
     3421 ggtgggcgtg ataggagccc atgtacggga ggtctgaggg gtacttgtac agagatgact
     3481 ccggggggtg gggctggagg gcctgggcga tcccgtggaa gtcgaacttg taggcgtagc
     3541 gcttcccatg gaccttggtc atgatgttct tgtcatagta gtaacggagg gcgcggctga
     3601 gcttatcgta gttcatgttg ggtttgctct tccgctctcc ccagcgccgg gccacctcgt
     3661 cgggatccgt catcttgaac tccccgttgg tgccttccca ggtgatgcag ctggagttgg
     3721 agctgtccga caggagctcc aggaggaact gccaaagctg gatctggcca ctgcctggat
     3781 ttgcaaggcg gctacttgtt ggtccaagaa tctgataagg atctaactga ggacgctggt
     3841 cttcagtttt gggcactgtg gaaggagatg gttgagcagc tttcgactgg ggcgtggggt
     3901 ggccgtgacc ggtccaggct gatctcctgg ggggctcata tggtaaatct ggcctagttg
     3961 taattctttg cgtagcttca ggatatactg aagtatttgg gaaaataaaa gctgcacccc
     4021 ctgtgtttct agcatgcatt aaccgtggag agttttgtaa ggctttatca acatcatctg
     4081 aagtcaaatg tggaagagga gtctctctga ggtagtggag atgtgagaga aggatgtcgg
     4141 cgttgtagct gggggtgagc ctctggaagt cgtccttggt catcttgcac agttccttcc
     4201 catcgatgtt ctggaataac aagatgttga cgtctggaag gccatattct ttcaccgccc
     4261 actccagcca ctgccgcaca tggtctgtac tccatagcgt aggatctgct ggcacgataa
     4321 ctctgcgctc gttcgtggtc atgtttgggg gtggcatgtg cttctcctcc atgtagctgc
     4381 cgtagttcat cccaacggtg tctgggctgc ccaccatctt cccgcctttg gccacactgc
     4441 attcatcagg agagttcctt gagccattca cctggctagg gttacattcc attttgatgg
     4501 tgaccctggc tgggggttga gacagccaat cctgctgagg gacgcgtggg ctcatcttgg
     4561 aagtctgtcc atagtcgctg gaggaggacg cggtcatctc tgtcttagcc aggtgtggcg
     4621 ttccgtaggc acactcaaac aacgactggt cctcactcac aactgataag gcttccttga
     4681 tatgagctgc tgggtccggg acagtctgaa tcatggtggc aacttttgta tacaaagttg
     4741 attctttacg agggtaggaa gtggtacgga aagttggtat aagacaaaag tgttgtggaa
     4801 ttgaagttta ctcaaaaaat cagcactctt ttataggcgc cctggtttac ataagcaaag
     4861 cttatacgtt ctctatcact gatagggagt aaactggata tacgttctct atcactgata
     4921 gggagtaaac tgtagatacg ttctctatca ctgataggga gtaaactggt catacgttct
     4981 ctatcactga tagggagtaa actccttata cgttctctat cactgatagg gagtaaagtc
     5041 tgcatacgtt ctctatcact gatagggagt aaactcttca tacgttctct atcactgata
     5101 gggagtaaac tcgagagaga aatgttctgg cacctgcact tgcactgggg acagcctatt
     5161 ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt
     5221 cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattc tcgaggtgat
     5281 aattccaccg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5341 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     5401 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5461 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5521 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     5581 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     5641 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     5701 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     5761 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     5821 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     5881 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     5941 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     6001 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     6061 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     6121 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     6181 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     6241 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     6301 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     6361 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     6421 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggggatca
     6481 tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc tggaattact
     6541 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     6601 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     6661 cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga
     6721 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     6781 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     6841 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     6901 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     6961 aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt
     7021 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     7081 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     7141 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     7201 tcttgacgat tttgaccttg acatgctccc cgggtaaatc tccagaggat cataatcagc
     7261 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7321 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     7381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgccccga
     7441 gcttcctcgc tcactgactc gctgcagcct gcttttttgt acaaacttgt gatgccgcca
     7501 ccgcggtagc actagttagc ttaattaacg agagcataat attgatatgt gccaaagttg
     7561 tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag ctaattactt
     7621 attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt tgtaaaagag
     7681 agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     7741 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     7801 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     7861 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     7921 ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg tgaataccga
     7981 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     8041 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8101 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8161 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8221 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8281 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8341 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8401 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8461 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8521 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8581 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     8641 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8701 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8761 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8821 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8881 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8941 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9001 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9061 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9121 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9181 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9241 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9301 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9361 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9421 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9481 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9541 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9601 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9661 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9721 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9781 caaaggatct tc
//
