LOCUS       1                      10074 bp    DNA     circular SYN 23-SEP-2025
DEFINITION  1.
ACCESSION   1
VERSION     1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        5576..6754
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="strong constitutive promoter for human
                     elongation factor EF-1α"
     CDS             6773..7519
                     /note="pLannotate"
                     /label="Tet-On® 3G"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     CDS             complement(3641..4996)
                     /note="pLannotate"
                     /label="FLI1_HUMAN"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Sequence-specific
                     transcriptional activator (PubMed:24100448,
                     PubMed:26316623, PubMed:28255014). Recognizes the DNA
                     sequence 5'-C[CA]GGAAGT-3'. From Homo sapiens (Human)."
     rep_origin      8458..8886
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis "
     promoter        complement(5027..5394)
                     /note="pLannotate"
                     /label="TRE3GS promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="3rd-generation Tet-responsive promoter that
                     can be activated by binding of Tet-On® 3G, modified to
                     eliminate binding sites for endogenous mammalian
                     transcription factors"
     CDS             9044..9904
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     CDS             complement(1861..2457)
                     /note="pLannotate"
                     /label="PUAC_STRAD"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Detoxification of puromycin.
                     From Streptomyces alboniger."
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     promoter        complement(2478..2977)
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="mouse phosphoglycerate kinase 1 promoter"
     polyA_signal    complement(1617..1738)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     promoter        8939..9043
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     misc_feature    complement(5398..5489)
                     /note="pLannotate"
                     /label="pause site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /description="RNA polymerase II transcriptional pause
                     signal from the human α2 globin gene"
     polyA_signal    7636..7717
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     polyA_signal    complement(3335..3390)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="rabbit β-globin polyadenylation signal (Gil
                     and Proudfoot, 1987)"
     polyA_signal    complement(5503..5551)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="synthetic polyadenylation signal"
     CDS             8296..8453
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
                     /description="LacZα fragment of β-galactosidase; lacZ
                     fragment"
     CDS             complement(3572..3613)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="epitope tag from simian virus 5"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for the E. coli lac operon"
     promoter        1490..1520
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for the E. coli lac operon"
     protein_bind    complement(7748..7772)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    3615..3639
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; recombination site
                     for the Gateway® BP reaction BP Clonase™"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="CAP binding activates transcription in the
                     presence of cAMP. E. coli catabolite activator protein"
     protein_bind    complement(5002..5022)
                     /note="pLannotate"
                     /label="attB5"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="mutant version of attB; core recombination
                     site for the Gateway® BP reaction BP Clonase™"
     promoter        complement(8275..8293)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T7 RNA polymerase"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="The lac repressor binds to the lac operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG). lac repressor
                     encoded by lacI"
     protein_bind    1528..1544
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="The lac repressor binds to the lac operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG). lac repressor
                     encoded by lacI"
     CDS             complement(1733..1858)
                     /note="pLannotate"
                     /label="BLE_KLEPN (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Binding protein with a strong
                     affinity to the bleomycin family of antibiotics. Binds to
                     CL990; an antimitotic-antibiotic compound. From Klebsiella
                     pneumoniae."
     CDS             complement(3461..3538)
                     /note="pLannotate"
                     /label="HBB_PONPY (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="72.2"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at transcript level:
                     Swiss-Prot protein existence level 2. Involved in oxygen
                     transport from the lung to the various peripheral tissues.
                     From Pongo pygmaeus (Bornean orangutan)."
     CDS             complement(1047..1178)
                     /note="pLannotate"
                     /label="FP25_NPVAC (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. From Autographa californica
                     nuclear polyhedrosis virus (AcMNPV)."
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
                     /description="lac repressor; lacI; The lac repressor binds
                     to the lac operator to inhibit transcription in E. coli.
                     This inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     repeat_region   7728..7739
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     repeat_region   complement(749..760)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     CDS             7530..7613
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
                     /description="large T antigen from SV40 (simian virus 40)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc tttagcgcgc
     1081 gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc tatgacgtgt
     1141 ttaaagttta ggtcgagtaa agcgcaaatc ttttttaacc ctagaaagat agtctgcgta
     1201 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
     1261 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
     1321 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
     1381 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
     1441 acttacgtga taacttatta tatatatatt ttcttgttat agatcaggct ttacacttta
     1501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1561 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     1621 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1681 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     1741 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     1801 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctctca
     1861 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
     1921 ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag
     1981 gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc
     2041 cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc
     2101 cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc
     2161 gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc
     2221 cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc
     2281 gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc
     2341 cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac
     2401 ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt
     2461 aagcttgggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac
     2521 gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg
     2581 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
     2641 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
     2701 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
     2761 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
     2821 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
     2881 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
     2941 gctccagacc gccttgggaa aagcgcctcc cctacccggt agaattatcg aattcctgca
     3001 ggtcgaggga tctccataag agaagaggga cagctatgac tgggagtagt caggagagga
     3061 ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata
     3121 acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg
     3181 aatggacagc agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca
     3241 tggcagccag catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt
     3301 ttaaatgatt tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca
     3361 ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca
     3421 tgatgtcccc ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg
     3481 cattggccac accagccacc accttctgat aggcagcctg cacctgagga gtgaattcga
     3541 tgggggatcc cttcgctaga gcggccatct acgtagaatc gagaccgagg agagggttag
     3601 ggataggctt acccaccact ttgtacaaga aagctgggta gtagtagctg cctaagtgtg
     3661 aaggcacgtg ggtgttagga tggcggggga cgttggggtt ggggtagatt ccccccgtgg
     3721 gggaggtcca gtattgtgat gcggctccaa agaagctgga ggaagtgaca ggcatggagg
     3781 atggatgggg agggacaaag ttcaccttct gctggtgggc atggtaggaa ggcatgtagg
     3841 agatgtcaga agggtacttg tacatggacg actcggtcgg atgtggctgc agagcctggg
     3901 caatgccgtg gaagtcaaat ttgtaagcat atcttttgcc gtgcactttg gtcataatgt
     3961 ttttatcata gtaataacgg agggcccggc tcagcttgtc gtaattcatg ttgggcttgc
     4021 ttttccgctc gccccagcgc ctggccacct catcggggtc cgtcattttg aactccccgt
     4081 tggtcccctc ccaggtgata cagctggcgt tggcgctgtc ggagagcagc tccaggagga
     4141 attgccacag ctggatctgc ccgcttccag ggttggctag gcgactgctg gtcgggccca
     4201 ggatctgata cggatctggc tggggccgtt gctctgtatt cttactgatc gtttgtgccc
     4261 ctccaagggg aggacttttg ttgaggccag aattcatgtt attgccccaa gctcctcttc
     4321 tgactgagtc ataagaaggg tcttctttga cactcaatcg tgaggattgg tcggtgtggg
     4381 aggttgtatt ataggccagc agtgaacttt ccctgaggta actgaggtgt gacaacagca
     4441 cttccgtgtt gtagagggtg gtggcgcgga ggaagtcctc cttgttcatt ttacacagtt
     4501 ccttgccatc catgttctgg aaaaaggatg tgtcgatctc catcaagctg tactccttta
     4561 tggcccactc cagccattgc ctcacatgct cctgtgtcca cagtgtgggg tctgcgggga
     4621 cgatgactct cctctcgttg gtggtcatgt tgggaggagg ggggccattc ttctcgtcca
     4681 tatagctgtt gtagttcatg gggttggact cgcctccgcc caccagcttg ctgcatttgc
     4741 taacgctgca gtccaccgga gactccctgg atccattcat gtggtcatac tcccgcttga
     4801 cgttgaccct cactggctga ttgatccact cctgctgtgg tgggaggggg ttgatcttgt
     4861 ggggctgccc gtagtcagga ctccccgagg cagtcatgtc ggccttgggg agatgggctg
     4921 ccgctccgta cgctgagtca aagagggact ggtcgtcgct caccaccgac agagcctcct
     4981 taatagtccc gtccatggtg gcaacttttg tatacaaagt tgattcttta cgagggtagg
     5041 aagtggtacg gaaagttggt ataagacaaa agtgttgtgg aattgaagtt tactcaaaaa
     5101 atcagcactc ttttataggc gccctggttt acataagcaa agcttatacg ttctctatca
     5161 ctgataggga gtaaactgga tatacgttct ctatcactga tagggagtaa actgtagata
     5221 cgttctctat cactgatagg gagtaaactg gtcatacgtt ctctatcact gatagggagt
     5281 aaactcctta tacgttctct atcactgata gggagtaaag tctgcatacg ttctctatca
     5341 ctgataggga gtaaactctt catacgttct ctatcactga tagggagtaa actcgagaga
     5401 gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt
     5461 tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac
     5521 acagatgtaa tgaaaataaa gatattttat tctcgaggtg ataattccac cgtgaggctc
     5581 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5641 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5701 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5761 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     5821 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     5881 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5941 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     6001 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     6061 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     6121 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6181 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6241 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6301 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6361 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6421 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6481 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6541 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6601 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6661 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6721 tcaaagtttt tttcttccat ttcaggtgtc gtgaggggat catcgaatta ccatgtctag
     6781 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga
     6841 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     6901 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     6961 tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     7021 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     7081 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     7141 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     7201 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     7261 cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga
     7321 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     7381 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     7441 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     7501 tgacatgctc cccgggtaaa tctccagagg atcataatca gccataccac atttgtagag
     7561 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7621 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7681 atcacaaatt tcacaaataa agcatttttt tcactgcccc gagcttcctc gctcactgac
     7741 tcgctgcagc ctgctttttt gtacaaactt gtgatgccgc caccgcggta gcactagtta
     7801 gcttaattaa cgagagcata atattgatat gtgccaaagt tgtttctgac tgactaataa
     7861 gtataatttg tttctattat gtataggtta agctaattac ttattttata atacaacatg
     7921 actgttttta aagtacaaaa taagtttatt tttgtaaaag agagaatgtt taaaagtttt
     7981 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
     8041 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     8101 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     8161 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aaataatagt
     8221 ttctaatttt tttattattc agcctgctgt cgtgaatacc gagctccaat tcgccctata
     8281 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     8461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8881 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
