LOCUS       attL3-tRNA-gRNA-2R_5_L_600-attL4        1183 bp    DNA     linear   UNA 26-JUL-2021
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...n-e5z0ggc
VERSION     urn.local...n-e5z0ggc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..415
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL3-tRNA-gRNA (concatenated sequence 1)"
     misc_feature    1..96
                     /note="Geneious type: attL3"
                     /standard_name="attL3"
     misc_feature    1..96
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL3 (concatenated sequence 1)"
     source          <97..>415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    97..415
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="U6-3-tRNA-gRNA-tRNA-3R-3.9 scaffold synth
                     (concatenated sequence 2)"
     misc_feature    <97..>119
                     /standard_name="eORI_mE_46"
     gene            120..191
                     /standard_name="tRNA:Ala-CGC-1-1"
     gene            complement(<120..>191)
                     /standard_name="gol"
     mRNA            complement(<120..>191)
                     /standard_name="gol-RE"
     mRNA            complement(<120..>191)
                     /standard_name="gol-RC"
     mRNA            complement(<120..>191)
                     /standard_name="gol-RD"
     mRNA            complement(<120..>191)
                     /standard_name="gol-RB"
     tRNA            120..191
                     /standard_name="tRNA:Ala-CGC-1-1-RA"
     exon            120..191
                     /standard_name="exon"
     misc_feature    121..211
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGC
                     GGCCCGGGTTCGATTCCCGGCCGATGCAGGGTCTTC"
     misc_feature    192..>211
                     /note="Geneious type: gRNA Target"
                     /standard_name="(Efficiency:8.50 Frameshift:44.6 OT:0
                     seedScore:13)"
     misc_RNA        212..287
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /standard_name="gRNA scaffold"
     tRNA            308..365
                     /standard_name="tRNA"
     gene            366..369
                     /standard_name="snRNA:U6:96Ac"
     regulatory      366..415
                     /note="Geneious type: terminator"
                     /standard_name="snRNA:U6:96Ac 3'"
     misc_feature    366..>415
                     /standard_name="eORI_mE_556"
     misc_feature    365^366
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="GAAGACCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGG
                     CTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC"
     misc_feature    416..1086
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="2R_5_L_600 (concatenated sequence 2)"
     misc_feature    416..1086
                     /note="Geneious type: Homology Arm"
                     /standard_name="2R_5_L"
     gene            <416..>1086
                     /standard_name="Ir41a"
     mRNA            <416..>1086
                     /standard_name="Ir41a-RC"
     CDS             469..>1086
                     /codon_start=2
                     /standard_name="CDS"
     exon            469..>1086
                     /standard_name="exon"
     misc_feature    837..1086
                     /note="Geneious type: Homology Arm"
                     /standard_name="2R_5_L_short"
     misc_feature    1087..1183
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL4 (concatenated sequence 3)"
     misc_feature    <1087..1183
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL4 (concatenated sequence 4)"
     misc_feature    complement(1087..1183)
                     /note="Geneious type: attL4"
                     /standard_name="attL4"
ORIGIN      
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
       61 gcttttttat aatgccaact ttgtataata aagttgcttt gagtgtgtgt agacatcaag
      121 gggacgtagc tcagtggtag agcgctcgct tcgcatgtga gaagtcccgg gttcaaaccc
      181 cggcgtctcc aggccatacg cgcttctgat ggttttagag ctagaaatag caagttaaaa
      241 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgctaa caaagcacca
      301 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
      361 gtgcattttt tgcctacctg gagcctgaga gttgttcaat aaaataaaaa tgtttaaaat
      421 tgttttttga tctgttgtta taaggaacct tctttattat ttttgtagct gccgcaaatg
      481 attggggcaa agtttatcca aatatgtctg gagatggtgc tcttggtatg cttattaatc
      541 gtaaagcaga tatatgcatt ggagctatgt actcgtggta cgaagattac acatacttag
      601 acctttctat gtatcttgta cgttctggaa taacgtgtct tgtaccagcg cctttgcggt
      661 tgactagttg gtaccttccc ttagagcctt tcaaagaaac tttatgggct gcaattctat
      721 tatgtctatg tgcagaagcc acaggattgg ttttagcata taaaagtgag caggcgctgt
      781 atgtactgcc tggctaccga gagggctggt ggacttgtac aagctttgga gtatgtacca
      841 cctttaaact tttcatatcg caatcaggaa acagcaaggc atattcactg acagttcgtg
      901 tactactctt tgcctgcttc cttaatgatt taataataac cagcatatat ggtggcggcc
      961 ttgctagtat attgacaatt cctagcatgg acgaggcagc cgacactgtc acccgcttgc
     1021 gatttcaccg attacagtgg gcggccaact cagaggcatg ggtctcggcc atacgcgctt
     1081 ctgatgcaac ttttctatac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1141 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttg
//
LOCUS       attL5-2R_5_R_600-attL6         790 bp    DNA     linear   UNA 26-JUL-2021
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...o-e5z0ggc
VERSION     urn.local...o-e5z0ggc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL5 (concatenated sequence 1)"
     misc_feature    1..97
                     /note="Geneious type: attL5"
                     /standard_name="attL5"
     misc_feature    98..693
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="2R_5_R_600 (concatenated sequence 2)"
     misc_feature    98..693
                     /note="Geneious type: Homology Arm"
                     /standard_name="2R_5_R"
     misc_feature    98..340
                     /note="Geneious type: Homology Arm"
                     /standard_name="2R_5_R_short"
     gene            <98..>693
                     /standard_name="Ir41a"
     mRNA            <98..>693
                     /standard_name="Ir41a-RC"
     CDS             join(<98..99,328..>693)
                     /codon_start=2
                     /standard_name="CDS"
     exon            <98..99
                     /standard_name="exon"
     CDS             159..273
                     /codon_start=1
                     /standard_name="CDS"
     exon            159..273
                     /standard_name="exon"
     exon            328..>693
                     /standard_name="exon"
     misc_feature    694..790
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL6 (concatenated sequence 3)"
     misc_feature    <694..790
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="attL6 (concatenated sequence 3)"
     misc_feature    complement(694..790)
                     /note="Geneious type: attL6"
                     /standard_name="attL6"
     misc_feature    693^694
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CCAGTTGATAGATGTTTCCACCT"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtataca aaagttgagg taagtgtttt aatgaagatc
      121 aatatcatct tagaagcata cgtttctttc tatgaaaggc attagtgaag gatatattgt
      181 acaattttca catctatagc gacgatgagt tgctacgctt agcacaggac cagcatatgc
      241 gcattggatt tactgtggag cgtctgccat tcggtaacaa caaaaattaa tgacgggaac
      301 caatattata tttttcttgt tctgtaggtc actttgctat cggaaactat ttggggcctc
      361 aagcgattga ccagttagtt ataatgaagg acgatattta ttttcaatat acggtggctt
      421 ttgttcccag actttggccc ctcctcgata aattaaatac tctgatatat agctggcatt
      481 cgtctggttt cgataaatac tgggaatatc gagttgttgc cgataactta aatctgaaga
      541 tacaacaaca agttcaagaa acaatgacag gaactaaaga tattggtcca gtcccgcttg
      601 gaatgtcaaa ctttgcggga tttataattg tctggatatt aggatctgct atagctacat
      661 taactttttt gttagaacta tcactgacat atacaacttt ttaatacaaa gttggcatta
      721 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      781 tcattatttg
//
