LOCUS       attL3-yaktan2sanHAL-5'pBac-attL4        1385 bp    DNA     linear   UNA 16-AUG-2022
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local.sternd.8f-e85cqt1
VERSION     urn.local.sternd.8f-e85cqt1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL3           1..97
                     /label="attL3"
     misc_feature    1..431
                     /Source="referenceToDocument: urn:local:sternd:75-e85cdw0"
                     /label="attL3-tRNA-gRNA-GCbalanced (concatenated sequence
                     1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2..97
                     /Source="referenceToDocument: urn:local:sternd:5g-d2c1bdk"
                     /label="attL3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     source          <98..>431
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    <98..>120
                     /created_by="sternd"
                     /label="eORI_mE_46"
     misc_feature    98..431
                     /Source="referenceToDocument: urn:local:sternd:5a-d2c1bdj"
                     /label="U6-3-tRNA-gRNA-tRNA-3R-3.9 scaffold synth
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     tRNA            121..192
                     /created_by="sternd"
                     /label="tRNA:Gln-CTG-2-4-RA"
     gene            121..192
                     /Source="FlyBase"
                     /ID="FBgn0051939"
                     /fullname="transfer RNA:Glutamine-CTG 2-4"
                     /Alias="FBgn0060106,AE002638.trna6-GlnCTG,tRNA:Q:CTG:AE002
                     638-c,CR31939,chr2L.trna39-GlnCTG,transfer
                     RNA:CR31939,tRNA:CR31939,tRNA-Gln-CTG-2-4,CAG"
                     /Ontology_term="SO:0000663,SO:0000087,GO:0033428,GO:000582
                     9,GO:0006412"
                     /Dbxref="FlyBase:FBan0031939,FlyBase_Annotation_IDs:CR3193
                     9,EntrezGene:3772443,RNAcentral:URS000017A2F3_7227,GenomeR
                     NAi:3772443"
                     /gbunit="AE014134"
                     /derived_computed_cyto="22B8-22B8"
                     /label="tRNA:Gln-CTG-2-4"
     mRNA            complement(<121..>192)
                     /Source="FlyBase"
                     /ID="FBtr0330672"
                     /Dbxref="FlyBase_Annotation_IDs:CG4238-RH,REFSEQ:NM_001272
                     966"
                     /label="CG4238-RH"
     tRNA            121..192
                     /Source="FlyBase"
                     /ID="FBtr0077862"
                     /Alias="CR31939-RA,tRNA:CR31939-RA"
                     /Dbxref="FlyBase_Annotation_IDs:CR31939-RA,RNAcentral:URS0
                     00017A2F3_7227"
                     /modified_by="sternd"
                     /label="tRNA:Gln-CTG-2-4-RA"
     mRNA            complement(<121..>192)
                     /Source="FlyBase"
                     /ID="FBtr0301068"
                     /Dbxref="FlyBase_Annotation_IDs:CG4238-RF,REFSEQ:NM_001103
                     583"
                     /label="CG4238-RF"
     gene            complement(<121..>192)
                     /Source="FlyBase"
                     /ID="FBgn0031384"
                     /Ontology_term="SO:0000010,SO:0000087,GO:0005737,GO:000651
                     1,GO:0005829,GO:0000209,GO:0045732,GO:0016567,GO:0004842,G
                     O:0061630,GO:0043161,GO:0043066"
                     /Dbxref="FlyBase:FBan0004238,FlyBase_Annotation_IDs:CG4238
                     ,GB_protein:ABV53602,GB_protein:ABV53601,GB_protein:AAF513
                     14,GB:AY113280,GB_protein:AAM29285,GB:BF502221,UniProt/TrE
                     MBL:Q9VQ67,INTERPRO:IPR000569,INTERPRO:IPR014756,INTERPRO:
                     IPR017868,INTERPRO:IPR035983,GB_protein:AGB92432,GB_protei
                     n:AGB92431,GB_protein:AGB92434,GB_protein:AGB92433,UniProt
                     /TrEMBL:M9PE16,EntrezGene:33377,UniProt/TrEMBL:A8DYT3,UniP
                     rot/TrEMBL:A8DYT2,INTERPRO:IPR013783,UniProt/TrEMBL:Q8MZA5
                     ,EPD:CG4238_1,EPD:CG4238_2,EPD:CG4238_3,Fly-FISH:CG4238,Ge
                     nomeRNAi:33377,iBeetle-Base:TC007799,KEGG_GENES:Dmel_CG423
                     8"
                     /gbunit="AE014134"
                     /derived_computed_cyto="22B8-22C1"
                     /label="CG4238"
     exon            121..192
                     /Source="FlyBase"
                     /label="exon"
     misc_feature    193..212
                     /Original_Bases="TCGTGATAGGTTAATATGCG"
                     /label="TCGTGATAGGTTAATATGCG"
                     /note="Geneious type: Editing History Replacement"
     Homology_Arm    complement(<193..201)
                     /created_by="sternd"
                     /label="HAR"
     region          complement(<193..>212)
                     /Source="RefSeq"
                     /ID="NC_052526.1:1..24674111"
                     /Dbxref="taxon:7245"
                     /chromosome="X"
                     /collection-date=1983
                     /country="Cote d'Ivoire"
                     /gbkey="Src"
                     /genome="chromosome"
                     /isolation-source="600 pooled females from a lab stock"
                     /mol_type="genomic DNA"
                     /sex="female"
                     /strain="Tai18E2"
                     /tissue-type="whole fly"
                     /label="X"
     CRISPR          193..>212
                     /Target_Sequence="GTAGCATACTTAAATTCCCA"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="GTAGCATACTTAAATTCCCATGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.593"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 6"
     Homology_Arm    202..>212
                     /created_by="sternd"
                     /label="HAL"
     misc_feature    complement(202..205)
                     /created_by="sternd"
                     /label="Target TTAA site"
     CRISPR          206..>212
                     /Target_Sequence="ATTCCCATGGCTGAGTGAAC"
                     /Protospacer_Adjacent_Motif_(PAM)="CGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATTCCCATGGCTGAGTGAACCGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.518"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 9"
     CRISPR          206..>212
                     /Target_Sequence="ATTCCCATGGCTGAGTGAAC"
                     /Protospacer_Adjacent_Motif_(PAM)="CGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATTCCCATGGCTGAGTGAACCGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.518"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 7"
     CRISPR          complement(209..>212)
                     /Target_Sequence="ATACCGGTTCACTCAGCCAT"
                     /Protospacer_Adjacent_Motif_(PAM)="GGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATACCGGTTCACTCAGCCATGGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.612"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 8"
     CRISPR          complement(209..>212)
                     /Target_Sequence="ATACCGGTTCACTCAGCCAT"
                     /Protospacer_Adjacent_Motif_(PAM)="GGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATACCGGTTCACTCAGCCATGGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.612"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 2"
     CRISPR          complement(210..>212)
                     /Target_Sequence="GATACCGGTTCACTCAGCCA"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="GATACCGGTTCACTCAGCCATGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.578"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 7"
     CRISPR          complement(210..>212)
                     /Target_Sequence="GATACCGGTTCACTCAGCCA"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="GATACCGGTTCACTCAGCCATGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.578"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 1"
     misc_RNA        213..288
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /modified_by="sternd"
                     /label="sgRNA scaffold"
     tRNA            309..381
                     /created_by="sternd"
                     /label="tRNA:Val-TAC-1-2-RA"
     gene            309..381
                     /Source="FlyBase"
                     /ID="FBgn0052421"
                     /fullname="transfer RNA:Valine-TAC 1-2"
                     /Alias="FBgn0011995,FBgn0011996,FBgn0060019,AE002602.trna1
                     2-ValTAC,valine tRNA,tRNA[Val][[3a]],transfer
                     RNA:val3a:64D,tRNA:val3a:64D,tRNA:V3a:64D,tRNA:V3a,tRNA:V:
                     TAC:AE002602-a,CR32421,chr3L.trna46-ValTAC,tRNA-Val[UAC],t
                     ransfer RNA:CR32421,tRNA:CR32421,tRNA-Val-TAC-1-2,GUA"
                     /Ontology_term="SO:0000663,SO:0000087,GO:0033451,GO:000582
                     9,GO:0006412"
                     /Dbxref="FlyBase:FBan0032421,FlyBase_Annotation_IDs:CR3242
                     1,GB:M25879,EntrezGene:3771988,RNAcentral:URS000043A2BD_72
                     27,GenomeRNAi:3771988"
                     /gbunit="AE014296"
                     /derived_computed_cyto="64C13-64C13,64D1-64D2"
                     /label="tRNA:Val-TAC-1-2"
     tRNA            309..381
                     /Source="FlyBase"
                     /ID="FBtr0077158"
                     /Alias="CR32421-RA,tRNA:CR32421-RA"
                     /Dbxref="FlyBase_Annotation_IDs:CR32421-RA,RNAcentral:URS0
                     00043A2BD_7227"
                     /modified_by="sternd"
                     /label="tRNA:Val-TAC-1-2-RA"
     exon            309..381
                     /Source="FlyBase"
                     /label="exon"
     terminator      382..431
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="snRNA:U6:96Ac 3'"
     misc_feature    382..>431
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="eORI_mE_556"
     misc_feature    431^432
                     /Original_Bases="CCACAAGACGTCGCCGGCCATCCCAGTCCCTCGTGGATTGG
                     CCCGAGGCCAAGGGC"
                     /label="CCACAAGACGTCGCCGGCCATCCCAGTCCCTCGTGGATTGGCCCGAGGCC
                     AAGGGC"
                     /note="Geneious type: Editing History Deletion"
     Homology_Arm    complement(432..979)
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="HAL"
     misc_feature    complement(<432..979)
                     /Source="referenceToDocument: urn:local:sternd:86-e85cplr"
                     /label="yak_tan_region - HAL (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     CDS             <432..539
                     /Source="Gnomon"
                     /ID="cds-XP_002101715.1"
                     /Dbxref="GeneID:6525895,Genbank:XP_002101715.1"
                     /gbkey="CDS"
                     /gene="LOC6525895"
                     /product="extensin"
                     /protein_id="XP_002101715.1"
                     /codon_start=1
                     /label="XP_002101715.1"
     exon            <432..652
                     /Source="Gnomon"
                     /ID="exon-XM_002101679.3-1"
                     /Dbxref="GeneID:6525895,Genbank:XM_002101679.3"
                     /gbkey="mRNA"
                     /gene="LOC6525895"
                     /product="extensin"
                     /transcript_id="XM_002101679.3"
                     /label="exon"
     gene            <432..652
                     /Source="Gnomon"
                     /ID="gene-LOC6525895"
                     /Dbxref="GeneID:6525895"
                     /gbkey="Gene"
                     /gene="LOC6525895"
                     /gene_biotype="protein_coding"
                     /label="LOC6525895"
     mRNA            <432..652
                     /Source="Gnomon"
                     /ID="rna-XM_002101679.3"
                     /Dbxref="GeneID:6525895,Genbank:XM_002101679.3"
                     /gbkey="mRNA"
                     /gene="LOC6525895"
                     /model_evidence="Supporting evidence includes similarity
                     to: 1 Protein, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments"
                     /product="extensin"
                     /transcript_id="XM_002101679.3"
                     /label="XM_002101679.3"
     region          <432..>979
                     /Source="RefSeq"
                     /ID="NC_052526.1:1..24674111"
                     /Dbxref="taxon:7245"
                     /chromosome="X"
                     /collection-date=1983
                     /country="Cote d'Ivoire"
                     /gbkey="Src"
                     /genome="chromosome"
                     /isolation-source="600 pooled females from a lab stock"
                     /mol_type="genomic DNA"
                     /sex="female"
                     /strain="Tai18E2"
                     /tissue-type="whole fly"
                     /label="X"
     CRISPR          complement(711..733)
                     /Target_Sequence="ACATTTAATTGATATTTCAA"
                     /Protospacer_Adjacent_Motif_(PAM)="AGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ACATTTAATTGATATTTCAAAGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.474"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 15"
     CRISPR          750..772
                     /Target_Sequence="TATAGTGCACAAACACCACT"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="TATAGTGCACAAACACCACTTGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.550"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 1"
     CRISPR          complement(765..787)
                     /Target_Sequence="CGATAAGTGGTTTTTCCAAG"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="CGATAAGTGGTTTTTCCAAGTGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.562"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 14"
     CRISPR          complement(778..800)
                     /Target_Sequence="TTTTTTTATTACTCGATAAG"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="TTTTTTTATTACTCGATAAGTGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.451"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 13"
     CRISPR          788..810
                     /Target_Sequence="AGTAATAAAAAAAATAGTTG"
                     /Protospacer_Adjacent_Motif_(PAM)="CGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="AGTAATAAAAAAAATAGTTGCGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.650"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 2"
     Enhancer        801..819
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="t_MSE1"
     misc_feature    811
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     CRISPR          complement(829..851)
                     /Target_Sequence="ATCATAATTTGTATATTCAT"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATCATAATTTGTATATTCATTGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.369"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 12"
     Enhancer        852..872
                     /created_by="sternd"
                     /label="t_MSE2"
     misc_feature    862
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CRISPR          881..903
                     /Target_Sequence="ACTAAAAATAAATATTCTAA"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ACTAAAAATAAATATTCTAATGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.459"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 4"
     CRISPR          887..909
                     /Target_Sequence="AATAAATATTCTAATGGTCC"
                     /Protospacer_Adjacent_Motif_(PAM)="AGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="AATAAATATTCTAATGGTCCAGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.508"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 5"
     CRISPR          complement(905..927)
                     /Target_Sequence="AAATAGGTGCATTTCACTCC"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="AAATAGGTGCATTTCACTCCTGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.519"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 11"
     CRISPR          complement(921..943)
                     /Target_Sequence="TTGAAATCAATAACAAAAAT"
                     /Protospacer_Adjacent_Motif_(PAM)="AGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="TTGAAATCAATAACAAAAATAGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.362"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 10"
     CRISPR          934..956
                     /Target_Sequence="TTGATTTCAACTTTAGATAC"
                     /Protospacer_Adjacent_Motif_(PAM)="CGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="TTGATTTCAACTTTAGATACCGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.444"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 6"
     CRISPR          949..971
                     /Target_Sequence="GATACCGGTTCACTCAGCCA"
                     /Protospacer_Adjacent_Motif_(PAM)="TGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="GATACCGGTTCACTCAGCCATGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.578"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 7"
     CRISPR          950..972
                     /Target_Sequence="ATACCGGTTCACTCAGCCAT"
                     /Protospacer_Adjacent_Motif_(PAM)="GGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATACCGGTTCACTCAGCCATGGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.612"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 8"
     CRISPR          complement(953..975)
                     /Target_Sequence="ATTCCCATGGCTGAGTGAAC"
                     /Protospacer_Adjacent_Motif_(PAM)="CGG"
                     /Enzyme="SpCas9"
                     /Recognition_pattern="ATTCCCATGGCTGAGTGAACCGG(-6/-6)"
                     /SpCas9="Cuts 3-4bp upstream of the PAM, displayed at 3bp"
                     /Doench_(2016)_Activity_Score="0.518"
                     /Doench_(2016)_Python_Version="3.9"
                     /Doench_(2016)_Algorithm_Version="0.2.4"
                     /label="CRISPR guide 9"
     misc_feature    976..979
                     /created_by="sternd"
                     /label="Target TTAA site"
     repeat_region   complement(980..1288)
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="5' pBac Optimal Transformation
                     Terminus"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hy-cha92nw"">5' pBac Optimal
                     Transformation Terminus</a>"
                     /label="5' pBac Optimal Transformation Terminus"
     misc_feature    979^980
                     /Original_Bases="TTAA"
                     /label="TTAA"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(980..>1288)
                     /note="piggyBac left arm"
                     /Transferred_From="piggyBac left arm"
                     /Transferred_Similarity="100.00%"
                     /label="piggyBac left arm"
     misc_feature    complement(980..1288)
                     /Source="referenceToDocument: urn:local:sternd:78-e85cdw0"
                     /label="5' pBac Optimal Transformation Terminus
                     (concatenated sequence 3) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1111^1112
                     /Original_Bases="GCCGCTTGGAGCTCCCGTGAGGCGTG"
                     /label="GCCGCTTGGAGCTCCCGTGAGGCGTG"
                     /note="Geneious type: Editing History Deletion"
     attL4           complement(1289..1385)
                     /label="attL4"
     misc_feature    complement(1289..1385)
                     /Source="referenceToDocument: urn:local:sternd:74-e85cdw0"
                     /label="attL4 (concatenated sequence 4) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1289..1385
                     /Source="referenceToDocument: urn:local:sternd:5d-d2c1bdk"
                     /label="attL4 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataat aaagttgctt tgagtgtgtg tagacatcaa
      121 ggttccatgg tgtaatggtt agcactcagg actctgaatc ctgcgatccg agttcaaatc
      181 tcggtggaac ctgtagcata cttaaattcc cagttttaga gctagaaata gcaagttaaa
      241 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcta acaaagcacc
      301 agtggtctgg ttccatagtg tagcggttat cacgtctgct ttacacgcag aaggtctccg
      361 gttcgatccc ggatggaacc attttttgcc tacctggagc ctgagagttg ttcaataaaa
      421 taaaaatgtt tttcaacagt gccgcgtcgg aaacgtacag tggcgtcgat agctacaatg
      481 tgggtcatgt ccagggctat ggatccagtg gctaccagta cagggggccc tatctgtagg
      541 tgtaccaaca gcccgcctaa tctaagatct atttattgca ttttttttcg tagcttcgta
      601 agttgtatta gtatcagttt taagatttaa gcgataaata aagaaatgtt aatagacact
      661 gaaagaaatg ttattacact tttcagaaaa taattgacac acgtcaaact cctttgaaat
      721 atcaattaaa tgttcatgtt ttctcagttt atagtgcaca aacaccactt ggaaaaacca
      781 cttatcgagt aataaaaaaa atagttgcgg aaataaaaaa gagactgtcc aatgaatata
      841 caaattatga tcttgaattc gaaaattggt tgatttaagc actaaaaata aatattctaa
      901 tggtccagga gtgaaatgca cctatttttg ttattgattt caactttaga taccggttca
      961 ctcagccatg ggaatttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
     1021 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
     1081 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
     1141 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
     1201 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
     1261 atatatatat tttcttgtta tagatatcca acttttctat acaaagttgg cattataaga
     1321 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1381 atttg
//
LOCUS       attL5-3'pBac-HARyaktan-attL6        1034 bp    DNA     linear   UNA 16-AUG-2022
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local.sternd.8l-e85d0te
VERSION     urn.local.sternd.8l-e85d0te
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /Source="referenceToDocument: urn:local:sternd:76-e85cdw0"
                     /label="attL5 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..97
                     /Source="referenceToDocument: urn:local:sternd:50-d2c1bdh"
                     /label="attL5 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     attL5           1..97
                     /label="attL5"
     misc_feature    98..337
                     /Source="referenceToDocument: urn:local:sternd:79-e85cdw0"
                     /label="3'pBac-modified (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     repeat_region   98..333
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3' pBac Optimal Transformation
                     Terminus"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hx-cha92nw"">3' pBac Optimal
                     Transformation Terminus</a>"
                     /label="3' pBac Optimal Transformation Terminus"
     misc_feature    <98..333
                     /created_by="User"
                     /Transferred_From="3' piggyBac arm"
                     /Transferred_Similarity="98.19%"
                     /modified_by="User"
                     /label="piggyBac 3' terminal repeat"
     misc_feature    98..337
                     /Source="referenceToDocument: urn:local:sternd:1w-e815yxz"
                     /label="3' pBac Optimal Transformation Terminus
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    101
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    139
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    145^146
                     /Original_Bases="TT"
                     /label="TT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    149
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    155
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    164
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    171
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    184..185
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    208..209
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    215
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    218
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    231
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    238..239
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    271..333
                     /created_by="sternd"
                     /label="3' repeat region"
     misc_feature    334..337
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(334..>337)
                     /Source="referenceToDocument: urn:local:sternd:78-e85cdw0"
                     /label="5' pBac Optimal Transformation Terminus
                     (concatenated sequence 3) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    338..937
                     /Source="referenceToDocument: urn:local:sternd:6n-e85cbeb"
                     /label="yak_tan_region - HAR (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     Homology_Arm    338..937
                     /created_by="sternd"
                     /label="HAR"
     region          <338..>937
                     /Source="RefSeq"
                     /ID="NC_052526.1:1..24674111"
                     /Dbxref="taxon:7245"
                     /chromosome="X"
                     /collection-date=1983
                     /country="Cote d'Ivoire"
                     /gbkey="Src"
                     /genome="chromosome"
                     /isolation-source="600 pooled females from a lab stock"
                     /mol_type="genomic DNA"
                     /sex="female"
                     /strain="Tai18E2"
                     /tissue-type="whole fly"
                     /label="X"
     exon            417..>937
                     /Source="Gnomon"
                     /ID="exon-XM_039372970.1-1"
                     /Dbxref="GeneID:6525896,Genbank:XM_039372970.1"
                     /gbkey="mRNA"
                     /gene="LOC6525896"
                     /product="gustatory receptor 8a, transcript variant X1"
                     /transcript_id="XM_039372970.1"
                     /label="exon"
     gene            417..>937
                     /Source="Gnomon"
                     /ID="gene-LOC6525896"
                     /Dbxref="GeneID:6525896"
                     /gbkey="Gene"
                     /gene="LOC6525896"
                     /gene_biotype="protein_coding"
                     /label="LOC6525896"
     exon            417..>937
                     /Source="Gnomon"
                     /ID="exon-XM_002101680.3-1"
                     /Dbxref="GeneID:6525896,Genbank:XM_002101680.3"
                     /gbkey="mRNA"
                     /gene="LOC6525896"
                     /product="gustatory receptor 8a, transcript variant X2"
                     /transcript_id="XM_002101680.3"
                     /label="exon"
     mRNA            417..>937
                     /Source="Gnomon"
                     /ID="rna-XM_039372970.1"
                     /Dbxref="GeneID:6525896,Genbank:XM_039372970.1"
                     /gbkey="mRNA"
                     /gene="LOC6525896"
                     /model_evidence="Supporting evidence includes similarity
                     to: 100% coverage of the annotated genomic feature by
                     RNAseq alignments, including 13 samples with support for
                     all annotated introns"
                     /product="gustatory receptor 8a, transcript variant X1"
                     /transcript_id="XM_039372970.1"
                     /label="XM_039372970.1"
     mRNA            417..>937
                     /Source="Gnomon"
                     /ID="rna-XM_002101680.3"
                     /Dbxref="GeneID:6525896,Genbank:XM_002101680.3"
                     /gbkey="mRNA"
                     /gene="LOC6525896"
                     /model_evidence="Supporting evidence includes similarity
                     to: 2 Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 8 samples with
                     support for all annotated introns"
                     /product="gustatory receptor 8a, transcript variant X2"
                     /transcript_id="XM_002101680.3"
                     /label="XM_002101680.3"
     CDS             610..>937
                     /Source="Gnomon"
                     /codon_start=1
                     /ID="cds-XP_039228904.1"
                     /Dbxref="GeneID:6525896,Genbank:XP_039228904.1"
                     /gbkey="CDS"
                     /gene="LOC6525896"
                     /product="gustatory receptor 8a isoform X1"
                     /protein_id="XP_039228904.1"
                     /label="XP_039228904.1"
     CDS             610..>937
                     /Source="Gnomon"
                     /codon_start=1
                     /ID="cds-XP_002101716.1"
                     /Dbxref="GeneID:6525896,Genbank:XP_002101716.1"
                     /gbkey="CDS"
                     /gene="LOC6525896"
                     /product="gustatory receptor 8a isoform X2"
                     /protein_id="XP_002101716.1"
                     /label="XP_002101716.1"
     misc_feature    complement(938..1034)
                     /Source="referenceToDocument: urn:local:sternd:77-e85cdw0"
                     /label="attL6 (concatenated sequence 4) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <938..1034
                     /Source="referenceToDocument: urn:local:sternd:4z-d2c1bdh"
                     /label="attL6 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     attL6           complement(938..1034)
                     /label="attL6"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtataca aaagttgtaa gagttttgtt actttataga
      121 agaaattttg agtttttggt tttttaatga ataagtaaac atacataaat ggtttgttga
      181 attccttatt agtatgtaag tgtaaatcca ataacacgta atatctattc gaattaacga
      241 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat gattatcttt
      301 aacgtacgtc acaatatgat tatctttcta gggttaagta tgctactaaa tgcaattatc
      361 ctaagccttc attctactta ttaaactata aaactataaa acgtagactg taggtacaat
      421 caatctgtag tttctggtta tttatatact acttataaat acggaaatcg agaaaggtac
      481 atggattacg gttctcggtg tatttgagaa aaccacttag gctaatggat tcgtcggtcc
      541 ttttaaaggg agattgaccc agggataagt actagccatc taaagggtct cgctttcgcg
      601 tagtcaacga tgagtggccg tctgggtcgg gttctgcagt tccacctgcg gctctaccag
      661 gtgctcggct tccatgggct gccgttgccg ggtgatggga atccggccag gactaggagg
      721 cgcctgatag cgtggagcct gttcgtgctc atttcgctga gtatcctcgt actggtgtgc
      781 cttttcagcg gcgatgagtt tctctatcgc ggcgacatgt tcggctgtgc caatgacgcc
      841 ctaaaatacg tattcgccga gttggccatg ctggccatat atctggagac gctgagcagc
      901 cagcggcatt tggccaactt ctggtgcctt cacttcacaa ctttttaata caaagttggc
      961 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1021 aaaatcatta tttg
//
