LOCUS       attL4_(reversed)          97 bp    DNA     linear   UNA 01-JUL-2021
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...63-hiscem7
VERSION     urn.local...63-hiscem7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    <1..97
                     /Source="referenceToDocument: urn:local:.:6s-hiscemj"
                     /label="attL4 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     attL4           complement(1..97)
                     /label="attL4"
ORIGIN      
        1 caacttttct atacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
       61 aacaggtcac tatcagtcaa aataaaatca ttatttg
//
LOCUS       attL3-tRNA-gRNA          415 bp    DNA     linear   UNA 25-MAR-2024
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...62-hiscem7
VERSION     urn.local...62-hiscem7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL3           1..96
                     /label="attL3"
     misc_feature    1..96
                     /Source="referenceToDocument: urn:local:.:6o-hisceme"
                     /label="attL3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    97..415
                     /Source="referenceToDocument: urn:local:.:6n-hisceme"
                     /label="U6-3-tRNA-gRNA-tRNA-3R-3.9 scaffold synth
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <97..>119
                     /created_by="sternd"
                     /label="eORI_mE_46"
     gene            120..191
                     /Source="FlyBase"
                     /ID="FBgn0050198"
                     /fullname="transfer RNA:Alanine-CGC 1-1"
                     /Alias="FBgn0060277,CR30198,AE002575.trna6-AlaCGC,tRNA:A:C
                     GC:AE002575-a,tRNA[[Ala]],chr2R.trna50-AlaCGC,tRNA-Ala-CGC
                     -1-1,transfer RNA:CR30198,tRNA:CR30198,GCG"
                     /Ontology_term="SO:0000663,SO:0000087,GO:0033456,GO:000582
                     9,GO:0006412"
                     /Dbxref="FlyBase:FBan0030198,FlyBase_Annotation_IDs:CR3019
                     8,EntrezGene:3772480,RNAcentral:URS0000067406_7227,GenomeR
                     NAi:3772480"
                     /gbunit="AE013599"
                     /derived_computed_cyto="60F3-60F3"
                     /label="tRNA:Ala-CGC-1-1"
     tRNA            120..191
                     /Source="FlyBase"
                     /ID="FBtr0072445"
                     /Alias="CR30198-RA,tRNA:CR30198-RA"
                     /Dbxref="FlyBase_Annotation_IDs:CR30198-RA,RNAcentral:URS0
                     000067406_7227"
                     /label="tRNA:Ala-CGC-1-1-RA"
     gRNA_Target     192..211
                     /created_by="sternd"
                     /label="gRNA target site (replace this with your target)"
     misc_RNA        212..287
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /modified_by="sternd"
                     /label="sgRNA scaffold"
     tRNA            308..365
                     /created_by="sternd"
                     /label="tRNA"
     terminator      366..415
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="snRNA:U6:96Ac 3'"
ORIGIN      
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
       61 gcttttttat aatgccaact ttgtataata aagttgcttt gagtgtgtgt agacatcaag
      121 gggacgtagc tcagtggtag agcgctcgct tcgcatgtga gaagtcccgg gttcaaaccc
      181 cggcgtctcc aaccctcatc ccggtcctaa tgttttagag ctagaaatag caagttaaaa
      241 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgctaa caaagcacca
      301 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
      361 gtgcattttt tgcctacctg gagcctgaga gttgttcaat aaaataaaaa tgttt
//
LOCUS       attL5                     97 bp    DNA     linear   UNA 01-JUL-2021
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...64-hiscem7
VERSION     urn.local...64-hiscem7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /Source="referenceToDocument: urn:local:.:6w-hisceml"
                     /label="attL5 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     attL5           1..97
                     /label="attL5"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtataca aaagttg
//
LOCUS       attL6_(reversed)          97 bp    DNA     linear   UNA 25-MAR-2024
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...65-hiscem7
VERSION     urn.local...65-hiscem7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL6           complement(1..97)
                     /label="attL6"
     misc_feature    <1..97
                     /Source="referenceToDocument: urn:local:.:70-hisceml"
                     /label="attL6 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
ORIGIN      
        1 caacttttta atacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
       61 aacaggtcac tatcagtcaa aataaaatca ttatttg
//
LOCUS       attL6-tRNA-gRNA_(reversed)         416 bp    DNA     linear   UNA 25-MAR-2024
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...o-hisd0l7
VERSION     urn.local...o-hisd0l7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..319
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     terminator      complement(1..50)
                     /created_by="sternd"
                     /modified_by="sternd"
                     /label="snRNA:U6:96Ac 3'"
     misc_feature    complement(1..319)
                     /Source="referenceToDocument: urn:local:.:s-hisd0l7"
                     /label="U6-3-tRNA-gRNA-tRNA-3R-3.9 scaffold synth
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     tRNA            complement(51..108)
                     /created_by="sternd"
                     /label="tRNA"
     misc_RNA        complement(129..204)
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /modified_by="sternd"
                     /label="sgRNA scaffold"
     gRNA_Target     complement(205..224)
                     /created_by="sternd"
                     /label="gRNA target site (replace this with your target)"
     gene            complement(225..296)
                     /Source="FlyBase"
                     /ID="FBgn0050198"
                     /fullname="transfer RNA:Alanine-CGC 1-1"
                     /Alias="FBgn0060277,CR30198,AE002575.trna6-AlaCGC,tRNA:A:C
                     GC:AE002575-a,tRNA[[Ala]],chr2R.trna50-AlaCGC,tRNA-Ala-CGC
                     -1-1,transfer RNA:CR30198,tRNA:CR30198,GCG"
                     /Ontology_term="SO:0000663,SO:0000087,GO:0033456,GO:000582
                     9,GO:0006412"
                     /Dbxref="FlyBase:FBan0030198,FlyBase_Annotation_IDs:CR3019
                     8,EntrezGene:3772480,RNAcentral:URS0000067406_7227,GenomeR
                     NAi:3772480"
                     /gbunit="AE013599"
                     /derived_computed_cyto="60F3-60F3"
                     /label="tRNA:Ala-CGC-1-1"
     tRNA            complement(225..296)
                     /Source="FlyBase"
                     /ID="FBtr0072445"
                     /Alias="CR30198-RA,tRNA:CR30198-RA"
                     /Dbxref="FlyBase_Annotation_IDs:CR30198-RA,RNAcentral:URS0
                     000067406_7227"
                     /label="tRNA:Ala-CGC-1-1-RA"
     misc_feature    complement(<297..>319)
                     /created_by="sternd"
                     /label="eORI_mE_46"
     misc_feature    <320..416
                     /Source="referenceToDocument: urn:local:.:r-hisd0l7"
                     /label="attL6 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     attL6           complement(320..416)
                     /label="attL6"
ORIGIN      
        1 aaacattttt attttattga acaactctca ggctccaggt aggcaaaaaa tgcaccagcc
       61 gggaatcgaa cccgggtctg taccgtggca gggtactatt ctaccactag accactggtg
      121 ctttgttagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta
      181 acttgctatt tctagctcta aaacattagg accgggatga gggttggaga cgccggggtt
      241 tgaacccggg acttctcaca tgcgaagcga gcgctctacc actgagctac gtccccttga
      301 tgtctacaca cactcaaagc aactttttaa tacaaagttg gcattataag aaagcattgc
      361 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttg
//
