LOCUS       (p109)_parental_cMYC_BB 8509 bp ds-DNA     circular     15-SEP-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    753..1613
                     /label="beta lactamase"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2840..3067
                     /label="RSV U3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     LTR             3068..3165
                     /label="R region"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     LTR             3166..3249
                     /label="U5 repeat"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     misc_feature    3257..4633
                     /label="Partial Gag/Pol"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     misc_feature    4638..5184
                     /label="cPPT"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     promoter        5195..6378
                     /label="EF1alpha Promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6391..6453
                     /label="CD8a LS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             6391..6489
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MALPVTALLLPLALLLHAARPGSEQKLISEEDL"
     misc_feature    6454..6489
                     /label="c-MYC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    6490..6514
                     /label="Insertion site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6515..6544
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="TTTPAPRPPT"
     exon            6515..6721
                     /label="CD8 Hinge and TM"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     CDS             6545..7183
                     /label="CD19 BBzeta CAR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="codon_start: 1 transl_table: 1 translation: MALPVTALLLPLALLLHAARPDIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVKLLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEITGGGGSGGGGSGGGGSEVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGLEWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGSYAMDYWGQGTSVTVSSTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYKQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR"
     CDS             6545..7183
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCKRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCELRVKFSRSADAPAYKQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR"
     exon            6722..6847
                     /label="4-1BB"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     exon            6848..7183
                     /label="CD3zeta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(7170..7186)
                     /label="PHU_33"
                     /note="sequence: tccctgtaataaacccgctagtcaCTCGAGttagcgagggggcaggg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             7184..7187
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="*"
     CDS             7187..7189
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="V"
     misc_feature    7188..7191
                     /label="SalI cut site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7194..7784
                     /label="Woodchuck PRE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             7921..8018
                     /label="R region"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
     LTR             8019..8102
                     /label="U5 repeat"
                     /ApEinfo_revcolor="#000000"
                     /ApEinfo_fwdcolor="#000000"
ORIGIN
        1 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      601 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      721 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      841 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      901 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      961 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1021 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1081 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1141 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1201 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1261 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1321 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1381 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1441 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1501 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1561 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1621 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2461 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2521 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2581 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2641 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2701 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2761 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     2821 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     2881 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     2941 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     3001 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     3061 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3241 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3301 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3361 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3421 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3481 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3541 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3601 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3661 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3721 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3781 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3841 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3901 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3961 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     4021 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     4081 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     4141 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     4201 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4261 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4321 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4381 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4441 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4501 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4561 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4621 atctcgacgg tatcgattag actgtagccc aggaatatgg cagctagatt gtacacattt
     4681 agaaggaaaa gttatcttgg tagcagttca tgtagccagt ggatatatag aagcagaagt
     4741 aattccagca gagacagggc aagaaacagc atacttcctc ttaaaattag caggaagatg
     4801 gccagtaaaa acagtacata cagacaatgg cagcaatttc accagtacta cagttaaggc
     4861 cgcctgttgg tgggcgggga tcaagcagga atttggcatt ccctacaatc cccaaagtca
     4921 aggagtaata gaatctatga ataaagaatt aaagaaaatt ataggacagg taagagatca
     4981 ggctgaacat cttaagacag cagtacaaat ggcagtattc atccacaatt ttaaaagaaa
     5041 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     5101 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5161 ggacagcaga gatccagttt ggctgcattg atcacgtgag gctccggtgc ccgtcagtgg
     5221 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     5281 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5341 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5401 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     5461 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     5521 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     5581 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     5641 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     5701 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     5761 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     5821 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     5881 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     5941 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     6001 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     6061 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     6121 gactccactg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     6181 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     6241 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     6301 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     6361 ccatttcagg tgtcgtgatc tagaggatcc atggccttac cagtgaccgc cttgctcctg
     6421 ccgctggcct tgctgctcca cgccgccagg ccggggtcag agcagaagct gatcagtgag
     6481 gaagacttgA AAAGCAGGTG aaaCACCTGC AAAAaccacg acgccagcgc cgcgaccacc
     6541 aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc
     6601 agcggcgggg ggcgcagtgc acacgagggg gctggacttc gcctgtgata tctacatctg
     6661 ggcgcccttg gccgggactt gtggggtcct tctcctgtca ctggttatca ccctttactg
     6721 caaacggggc agaaagaaac tcctgtatat attcaaacaa ccatttatga gaccagtaca
     6781 aactactcaa gaggaagatg gctgtagctg ccgatttcca gaagaagaag aaggaggatg
     6841 tgaactgaga gtgaagttca gcaggagcgc agacgccccc gcgtacaagc agggccagaa
     6901 ccagctctat aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag
     6961 acgtggccgg gaccctgaga tggggggaaa gccgagaagg aagaaccctc aggaaggcct
     7021 gtacaatgaa ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg
     7081 cgagcgccgg aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa
     7141 ggacacctac gacgcccttc acatgcaggc cctgccccct cgctaagtcg acaatcaacc
     7201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7561 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7741 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     7801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7861 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7921 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7981 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8041 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8101 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8161 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8401 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8461 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattac
//